Traditional biotechnological preparation of ethanol, ingredients and procedures?
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Aug 6
Traditional biotechnological preparation of ethanol involves the fermentation of sugars by yeast. The ingredients and procedures for this process are as follows:
Ingredients: 1. Sugar source (such as molasses, corn, or sugarcane) 2. Yeast (Saccharomyces cerevisiae) 3. Water
Procedures: 1. The first step in the traditional biotechnological preparation of ethanol is to prepare a fermentation medium by mixing the sugar source with water. The ratio of sugar to water will depend on the specific recipe being used.
2. The fermentation medium is then sterilized to kill off any unwanted microorganisms that could compete with the yeast during fermentation.
3. Once the fermentation medium has cooled to a suitable temperature, the yeast is added. The yeast will begin to metabolize the sugars in the medium, producing ethanol and carbon dioxide as byproducts.
4. The fermentation process typically takes several days to complete, during which time the mixture is stirred or agitated to ensure that the yeast is evenly distributed and has access to all of the sugars in the medium.
5. Once fermentation is complete, the mixture is allowed to settle, and the ethanol is separated from the remaining liquid through distillation.
6. The ethanol can then be further purified through processes such as filtration or distillation to remove any impurities and increase its concentration.
7. The final product is ethanol, which can be used as a fuel additive, solvent, or in the production of alcoholic beverages.
1. Mash Preparation: - Grind or crush the sugar source to release the fermentable sugars. - Mix the crushed material with water to form a mash. - Add nutrients to support yeast growth.
2. Fermentation: - Add yeast to the mash. - Incubate the mash at an optimal temperature (typically 25-30°C) for 2-5 days. - Yeast metabolizes the sugars in the mash, producing ethanol and carbon dioxide.
3. Distillation: - The fermented mash is heated, and the ethanol evaporates at a lower temperature than water. - The ethanol vapor is condensed and collected to yield a crude ethanol solution.
4. Rectification: - The crude ethanol undergoes a second distillation to purify it further. - Additional impurities are removed, and the ethanol concentration is increased to over 95%.
Steps in Traditional Ethanol Preparation:
1. Mash preparation: Grind or crush the sugar source and mix it with water to form a mash. 2. Temperature control: Maintain the fermentation temperature within the optimal range for yeast growth. 3. Nutrient supplementation: Add nutrients to the mash to support yeast metabolism and prevent contamination. 4. Agitation: Stir or aerate the mash occasionally to provide oxygen for yeast respiration. 5. Fermentation time: Allow the mash to ferment for the appropriate amount of time to maximize ethanol production. 6. Distillation: Heat the fermented mash to vaporize the ethanol and condense it to separate it from the water. 7. Rectification: Perform a second distillation to purify the ethanol and increase its concentration. 8. Storage: Store the purified ethanol in a sealed container to prevent evaporation or contamination.