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Aug 6
The procedure for Acid-fast bacilli (AFB) staining involves the following steps:
1. Collect a sputum sample or other specimen from the patient suspected of having a mycobacterial infection.
2. Prepare a thin smear of the specimen on a glass slide and allow it to air dry.
3. Fix the smear by passing it through a flame or using a fixative such as methanol.
4. Stain the smear using the Ziehl-Neelsen method or the Kinyoun method. These methods involve staining the smear with a primary stain (carbolfuchsin), decolorizing with acid-alcohol, and counterstaining with a contrasting color (methylene blue).
5. Examine the stained smear under a microscope using oil immersion at 1000x magnification. Acid-fast bacilli will appear as bright red or pink rods against a blue background.
6. Interpret the results based on the presence or absence of acid-fast bacilli in the smear. Positive results indicate the presence of mycobacteria, while negative results suggest the absence of mycobacteria.
7. Report the results to the healthcare provider for further diagnosis and treatment.
1. Collect a sputum sample or other specimen from the patient suspected of having a mycobacterial infection.
2. Prepare a thin smear of the specimen on a glass slide and allow it to air dry.
3. Fix the smear by passing it through a flame or using a fixative such as methanol.
4. Stain the smear using the Ziehl-Neelsen method or the Kinyoun method. These methods involve staining the smear with a primary stain (carbolfuchsin), decolorizing with acid-alcohol, and counterstaining with a contrasting color (methylene blue).
5. Examine the stained smear under a microscope using oil immersion at 1000x magnification. Acid-fast bacilli will appear as bright red or pink rods against a blue background.
6. Interpret the results based on the presence or absence of acid-fast bacilli in the smear. Positive results indicate the presence of mycobacteria, while negative results suggest the absence of mycobacteria.
7. Report the results to the healthcare provider for further diagnosis and treatment.
0
Google Gemini AI
Aug 6
Procedure of AFB (Acid-Fast Bacillus) Staining
Materials:
- Sample (e.g., sputum, tissue section)
- AFB staining kit
- Microscope
Steps:
1. Preparation of Smear:
- Create a thin smear of the sample on a glass slide.
- Allow it to air dry completely.
2. Heat Fixing:
- Gently pass the slide over a flame to fix the smear.
3. Primary Stain (Carbol Fuchsin):
- Flood the slide with carbol fuchsin.
- Heat the slide gently to boiling for 5-10 minutes.
4. Decolorizing (Acid-Alcohol):
- Wash the slide with water.
- Flood the slide with 3% hydrochloric acid in 95% ethanol (acid-alcohol) for 2-3 minutes.
- Rinse the slide with water.
5. Counter Stain (Methylene Blue):
- Flood the slide with methylene blue for 1-2 minutes.
- Rinse the slide with water.
6. Drying:
- Blot the slide gently to remove excess water.
- Allow it to air dry completely.
7. Examination:
- Examine the smear under a microscope using the oil immersion objective.
Interpretation:
- AFB-positive: Red or pink rods with an acid-fastness property (retains the primary stain after decolorizing).
- AFB-negative: Blue or green rods that do not retain the primary stain.
Quality Control:
- Include a known positive and negative control slide for verification.
- Ensure that the reagents are of good quality and not contaminated.
- Follow the procedure precisely, as any variations can affect the results.
Materials:
- Sample (e.g., sputum, tissue section)
- AFB staining kit
- Microscope
Steps:
1. Preparation of Smear:
- Create a thin smear of the sample on a glass slide.
- Allow it to air dry completely.
2. Heat Fixing:
- Gently pass the slide over a flame to fix the smear.
3. Primary Stain (Carbol Fuchsin):
- Flood the slide with carbol fuchsin.
- Heat the slide gently to boiling for 5-10 minutes.
4. Decolorizing (Acid-Alcohol):
- Wash the slide with water.
- Flood the slide with 3% hydrochloric acid in 95% ethanol (acid-alcohol) for 2-3 minutes.
- Rinse the slide with water.
5. Counter Stain (Methylene Blue):
- Flood the slide with methylene blue for 1-2 minutes.
- Rinse the slide with water.
6. Drying:
- Blot the slide gently to remove excess water.
- Allow it to air dry completely.
7. Examination:
- Examine the smear under a microscope using the oil immersion objective.
Interpretation:
- AFB-positive: Red or pink rods with an acid-fastness property (retains the primary stain after decolorizing).
- AFB-negative: Blue or green rods that do not retain the primary stain.
Quality Control:
- Include a known positive and negative control slide for verification.
- Ensure that the reagents are of good quality and not contaminated.
- Follow the procedure precisely, as any variations can affect the results.
0
Usmaan Milkeso 123
Aug 28
Principles
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