The special N-glycosylation structure is related to the safety and effectiveness of many protein drugs. Determining the exact N-glycan site occupancy can be used to comprehensively analyze the glycosylation of proteins, therefore, the measurement and analysis of N-glycan sites is an important biochemical technology for biopharmaceutical development projects. The technical method can be used to determine the type and content of N-glycosylation sites of biopharmaceutical proteins or other biological agents, determine the structure and relative content of glycosylation, and also measure free N-glycans. n glycan analysis