State the factors that affect enzyme catalyzed reaction? Describe an experiment to show how each other factors you have stated affect the rate of reaction? ?

The factors that affect enzyme-catalyzed reactions include temperature, pH, substrate concentration, enzyme concentration, and the presence of inhibitors or activators.

To demonstrate the effect of temperature on the rate of reaction, one could set up an experiment using the enzyme catalase and hydrogen peroxide. The reaction between catalase and hydrogen peroxide produces oxygen gas, which can be measured as an indicator of the reaction rate.

1. Set up several test tubes, each containing the same amount of catalase solution.
2. Vary the temperature of each test tube by placing them in water baths at different temperatures (e.g., 0°C, 25°C, 37°C, 50°C).
3. Add the same amount of hydrogen peroxide to each test tube and start a timer.
4. Measure the volume of oxygen gas produced at regular intervals (e.g., every 30 seconds) using a gas syringe.
5. Record the data and calculate the rate of reaction for each temperature.

The results of this experiment would show that the rate of the enzyme-catalyzed reaction increases with temperature up to a certain point (the optimal temperature), beyond which the reaction rate decreases. This demonstrates the effect of temperature on enzyme activity.

Similarly, one could design experiments to investigate the effects of pH, substrate concentration, enzyme concentration, and the presence of inhibitors or activators on enzyme-catalyzed reactions. Each experiment would involve manipulating the specific factor while keeping other variables constant, and measuring the rate of reaction as an indicator of enzyme activity.