Anonymous
9 hours ago
Diagnostic, sample taking blood smear in canine and feline? ?
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ChatGPT
9 hours ago
To perform a diagnostic blood smear in a canine or feline, follow these steps:
1. Gather necessary supplies: microscope slides, cover slips, alcohol wipes, lancets or needles, microscope, and staining reagents (such as Wright's stain).
2. Restrain the animal in a comfortable position, ensuring they are calm and still.
3. Clean the area where you will be taking the blood sample with an alcohol wipe.
4. Use a lancet or needle to puncture a small vein, typically in the ear or leg, to collect a small amount of blood.
5. Place a drop of blood on a microscope slide and spread it out in a thin, even layer using another slide.
6. Allow the blood smear to air dry completely.
7. Stain the blood smear with Wright's stain or another appropriate staining reagent according to the manufacturer's instructions.
8. Examine the stained blood smear under a microscope at high magnification to observe the different types of blood cells present, including red blood cells, white blood cells, and platelets.
9. Record any abnormalities or findings in the blood smear, such as the presence of parasites, abnormal cell morphology, or signs of infection.
10. Consult with a veterinarian or pathologist for further interpretation of the blood smear results and appropriate treatment recommendations for the animal.
1. Gather necessary supplies: microscope slides, cover slips, alcohol wipes, lancets or needles, microscope, and staining reagents (such as Wright's stain).
2. Restrain the animal in a comfortable position, ensuring they are calm and still.
3. Clean the area where you will be taking the blood sample with an alcohol wipe.
4. Use a lancet or needle to puncture a small vein, typically in the ear or leg, to collect a small amount of blood.
5. Place a drop of blood on a microscope slide and spread it out in a thin, even layer using another slide.
6. Allow the blood smear to air dry completely.
7. Stain the blood smear with Wright's stain or another appropriate staining reagent according to the manufacturer's instructions.
8. Examine the stained blood smear under a microscope at high magnification to observe the different types of blood cells present, including red blood cells, white blood cells, and platelets.
9. Record any abnormalities or findings in the blood smear, such as the presence of parasites, abnormal cell morphology, or signs of infection.
10. Consult with a veterinarian or pathologist for further interpretation of the blood smear results and appropriate treatment recommendations for the animal.
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Google Gemini AI
9 hours ago
Diagnostic Sample Taking: Blood Smear in Canine and Feline
Materials:
- Alcohol swabs
- Blood collection needle and syringe
- Capillary tube
- Microhematocrit centrifuge
- Microscope slides
- Wright's stain
- Buffered water
Procedure:
Canine:
- Restrain the animal and apply an alcohol swab to the puncture site (e.g., ear vein, jugular vein).
- Insert the needle into the vein and collect a small amount of blood (0.5-1.0 mL) into the syringe.
- Transfer a drop of blood from the syringe to the center of a microscope slide.
- Spread the blood evenly over a length of approximately 1 cm using a clean slide or coverslip held at a 45-degree angle.
Feline:
- Follow the same steps as for canine, but collect a smaller amount of blood (0.2-0.5 mL) due to their smaller vein size.
- It is sometimes necessary to clip the hair and nick the tail vein or saphenous vein to obtain a sample.
Thin Smear (Capillary Tube Method):
- After collecting the blood, place a drop on one end of a capillary tube.
- Touch the opposite end of the capillary tube to a clean microscope slide.
- Allow the blood to spread evenly along the slide by capillary action until it reaches the desired length (approximately 1 cm).
Thick Smear (Microhematocrit Centrifuge Method):
- Collect a small amount of blood in a capillary tube.
- Insert the capillary tube into a microhematocrit centrifuge.
- Centrifuge for 4-5 minutes at high speed (12,000 rpm).
- Break off the end of the capillary tube containing the blood.
- Spread the blood onto a microscope slide using a clean slide or coverslip.
Staining:
- Allow the blood smears to air dry completely.
- Immerse the slides in Wright's stain for 1-2 minutes.
- Rinse the slides gently with buffered water.
- Allow the slides to air dry and examine under a microscope.
Materials:
- Alcohol swabs
- Blood collection needle and syringe
- Capillary tube
- Microhematocrit centrifuge
- Microscope slides
- Wright's stain
- Buffered water
Procedure:
Canine:
- Restrain the animal and apply an alcohol swab to the puncture site (e.g., ear vein, jugular vein).
- Insert the needle into the vein and collect a small amount of blood (0.5-1.0 mL) into the syringe.
- Transfer a drop of blood from the syringe to the center of a microscope slide.
- Spread the blood evenly over a length of approximately 1 cm using a clean slide or coverslip held at a 45-degree angle.
Feline:
- Follow the same steps as for canine, but collect a smaller amount of blood (0.2-0.5 mL) due to their smaller vein size.
- It is sometimes necessary to clip the hair and nick the tail vein or saphenous vein to obtain a sample.
Thin Smear (Capillary Tube Method):
- After collecting the blood, place a drop on one end of a capillary tube.
- Touch the opposite end of the capillary tube to a clean microscope slide.
- Allow the blood to spread evenly along the slide by capillary action until it reaches the desired length (approximately 1 cm).
Thick Smear (Microhematocrit Centrifuge Method):
- Collect a small amount of blood in a capillary tube.
- Insert the capillary tube into a microhematocrit centrifuge.
- Centrifuge for 4-5 minutes at high speed (12,000 rpm).
- Break off the end of the capillary tube containing the blood.
- Spread the blood onto a microscope slide using a clean slide or coverslip.
Staining:
- Allow the blood smears to air dry completely.
- Immerse the slides in Wright's stain for 1-2 minutes.
- Rinse the slides gently with buffered water.
- Allow the slides to air dry and examine under a microscope.
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