Briefly outline the producer you would follow the mounting to observe cells from onion and a high power of a light microscope?

To observe cells from an onion under a light microscope at high power, you would follow the mounting process as follows:

1. Peel off a thin layer of the onion skin using forceps or a scalpel.
2. Place the thin layer of onion skin on a clean glass slide.
3. Add a drop of water or a drop of iodine solution to the onion skin to help make the cells more visible.
4. Gently place a coverslip over the onion skin, being careful not to trap any air bubbles.
5. Use the fine adjustment knob on the microscope to focus on the onion cells at low power.
6. Once the cells are in focus, switch to high power to observe the cells in more detail.

By following these steps, you should be able to observe the cells from an onion under a light microscope at high power.

Procedure:

1. Prepare Onion Root Tip:
- Remove the root tip from an onion.
- Fix the root tip in formaldehyde for at least 12 hours.
- Wash the root tip with water and store it in 70% ethanol.

2. Prepare Slides:
- Cut a thin section (about 5-10 µm) from the root tip using a microtome.
- Stain the section with safranin or methylene blue for 1-2 minutes.

3. Mount the Slide:
- Place a drop of immersion oil on the center of a clean glass slide.
- Carefully transfer the stained section onto the oil.
- Cover the section with a cover slip.

4. Observe Cells:
- Locate the onion root tip cells under a low-power objective (e.g., 10x).
- Adjust the focus until the cells are clear.
- Switch to a high-power objective (e.g., 40x or 100x) and adjust the fine focus for a clear view.
- Observe the cell components, including the nucleus, cell membrane, and cytoplasm.