When are Western blot results interpreted as indeterminate?

Western blot results are interpreted as indeterminate when there is not a clear presence or absence of specific bands corresponding to the target proteins being analyzed. This can occur when there is weak or faint band intensity, non-specific binding of antibodies, or overlapping bands that make it difficult to accurately interpret the results. In these cases, further testing or optimization of the experimental conditions may be necessary to obtain a conclusive result.