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Jul 25
1. Hematoxylin and eosin (H&E) staining: This is a commonly used staining technique in histology that uses hematoxylin to stain cell nuclei blue and eosin to stain cytoplasm and extracellular matrix pink.
2. Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue samples. The antibodies are labeled with a chromogen that produces a colored reaction when bound to the target protein.
3. Fluorescent staining: This technique uses fluorescent dyes or antibodies to label specific structures or proteins in cells or tissues. The labeled structures can then be visualized under a fluorescence microscope.
4. Periodic acid-Schiff (PAS) staining: This staining technique is used to detect carbohydrates, glycogen, and mucosubstances in tissues. It involves the oxidation of carbohydrates by periodic acid, followed by staining with Schiff reagent.
5. Giemsa staining: This staining technique is commonly used to visualize blood cells and microorganisms. It involves staining with a mixture of methylene blue and eosin, which produces a purple color in the nuclei and a pink color in the cytoplasm.
6. Gram staining: This staining technique is used to differentiate between Gram-positive and Gram-negative bacteria based on differences in cell wall composition. It involves staining with crystal violet, iodine, alcohol, and safranin.
7. Wright-Giemsa staining: This staining technique is commonly used to visualize blood cells, especially for the diagnosis of blood disorders. It involves staining with a mixture of Wright stain and Giemsa stain, which produces distinct colors in different blood cell types.
2. Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue samples. The antibodies are labeled with a chromogen that produces a colored reaction when bound to the target protein.
3. Fluorescent staining: This technique uses fluorescent dyes or antibodies to label specific structures or proteins in cells or tissues. The labeled structures can then be visualized under a fluorescence microscope.
4. Periodic acid-Schiff (PAS) staining: This staining technique is used to detect carbohydrates, glycogen, and mucosubstances in tissues. It involves the oxidation of carbohydrates by periodic acid, followed by staining with Schiff reagent.
5. Giemsa staining: This staining technique is commonly used to visualize blood cells and microorganisms. It involves staining with a mixture of methylene blue and eosin, which produces a purple color in the nuclei and a pink color in the cytoplasm.
6. Gram staining: This staining technique is used to differentiate between Gram-positive and Gram-negative bacteria based on differences in cell wall composition. It involves staining with crystal violet, iodine, alcohol, and safranin.
7. Wright-Giemsa staining: This staining technique is commonly used to visualize blood cells, especially for the diagnosis of blood disorders. It involves staining with a mixture of Wright stain and Giemsa stain, which produces distinct colors in different blood cell types.
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Google Gemini AI
Jul 25
Simple Staining
- Positive staining: Dyes stain specific cellular components or microorganisms.
- Negative staining: Dyes stain the background, leaving the target unstained.
Differential Staining
- Gram staining: Differentiates between Gram-positive and Gram-negative bacteria.
- Ziehl-Neelsen staining: Stains acid-fast bacteria, such as Mycobacterium tuberculosis.
- H&E staining: Stains tissue sections to differentiate between cell types.
Fluorescent Staining
- Immunofluorescence: Uses fluorescent antibodies to target specific proteins or molecules.
- FISH (fluorescent in situ hybridization): Hybridizes fluorescent probes to DNA or RNA sequences.
- Flow cytometry: Analyzes cells based on their fluorescence properties.
Cytochemical Staining
- PAS (periodic acid-Schiff) staining: Stains carbohydrates and glycoproteins.
- Sudan staining: Stains lipids.
- Enzyme histochemistry: Detects the presence of specific enzymes within cells.
Special Stains
- Papanicolaou (Pap) staining: Used to detect cervical cancer.
- Wright-Giemsa staining: Stains blood cells to differentiate between different types.
- Warthin-Starry staining: Stains Spirochetes.
Multiple Staining
- May-Grünwald-Giemsa staining: Combines simple and differential staining for blood smears.
- Gomori's trichrome staining: Differentiates between collagen, muscle, and other tissue components.
- Masson's trichrome staining: Stains connective tissue and muscle.
- Positive staining: Dyes stain specific cellular components or microorganisms.
- Negative staining: Dyes stain the background, leaving the target unstained.
Differential Staining
- Gram staining: Differentiates between Gram-positive and Gram-negative bacteria.
- Ziehl-Neelsen staining: Stains acid-fast bacteria, such as Mycobacterium tuberculosis.
- H&E staining: Stains tissue sections to differentiate between cell types.
Fluorescent Staining
- Immunofluorescence: Uses fluorescent antibodies to target specific proteins or molecules.
- FISH (fluorescent in situ hybridization): Hybridizes fluorescent probes to DNA or RNA sequences.
- Flow cytometry: Analyzes cells based on their fluorescence properties.
Cytochemical Staining
- PAS (periodic acid-Schiff) staining: Stains carbohydrates and glycoproteins.
- Sudan staining: Stains lipids.
- Enzyme histochemistry: Detects the presence of specific enzymes within cells.
Special Stains
- Papanicolaou (Pap) staining: Used to detect cervical cancer.
- Wright-Giemsa staining: Stains blood cells to differentiate between different types.
- Warthin-Starry staining: Stains Spirochetes.
Multiple Staining
- May-Grünwald-Giemsa staining: Combines simple and differential staining for blood smears.
- Gomori's trichrome staining: Differentiates between collagen, muscle, and other tissue components.
- Masson's trichrome staining: Stains connective tissue and muscle.
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