Outline the process of Tyndallization.

1. The first step in the process of Tyndallization is to place the substance to be sterilized in a container, such as a flask or test tube.

2. The container is then placed in a water bath and heated to a temperature of around 100°C for a period of time, typically 30 minutes to an hour. This heat kills off any vegetative bacteria present in the substance.

3. After the initial heating, the container is allowed to cool down to room temperature. This allows any spores present in the substance to germinate into vegetative bacteria.

4. The container is then heated again to 100°C for another period of time, typically 30 minutes to an hour. This second round of heating kills off the newly germinated vegetative bacteria.

5. The process of heating, cooling, and reheating is repeated for a total of three cycles. This ensures that any spores present in the substance have had a chance to germinate and be killed off.

6. After the final cycle, the substance is considered to be sterilized and can be safely used in laboratory experiments or other applications.