Mention 5 laboratory procedure that can be used to identify purity of a substance?
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Nov 22, 2023
1. Melting point determination: This procedure involves measuring the temperature at which a substance changes from a solid to a liquid state. A pure substance will have a sharp and well-defined melting point, while impurities can cause a depression or broadening of the melting point range.
2. Boiling point determination: Similar to melting point determination, this procedure involves measuring the temperature at which a substance changes from a liquid to a gas state. A pure substance will have a specific and consistent boiling point, while impurities can cause the boiling point to increase or the boiling range to broaden.
3. Chromatography: This technique separates the components of a mixture based on their different affinities for a stationary phase and a mobile phase. By comparing the separation pattern of a sample with that of a pure substance, the presence of impurities can be detected.
4. Spectroscopy: Various spectroscopic techniques, such as infrared spectroscopy, ultraviolet-visible spectroscopy, and nuclear magnetic resonance spectroscopy, can be used to analyze the absorption or emission of electromagnetic radiation by a substance. These techniques can provide information about the molecular structure and functional groups present, helping to identify impurities.
5. Elemental analysis: This procedure involves determining the elemental composition of a substance. Techniques such as elemental analysis by combustion or spectroscopic methods like X-ray fluorescence can be used to quantify the amounts of different elements present. Deviations from the expected elemental composition can indicate the presence of impurities.