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Describe an experiment that could be conducted to show that germinating been seed contains enzymes that digests starch?
Describe an experiment that could be conducted to show that germinating been seed contains enzymes that digests starch?
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Mar 8
One experiment that could be conducted to show that germinating bean seeds contain enzymes that digest starch is as follows:
1. Obtain a batch of bean seeds and soak them in water overnight to initiate the germination process.
2. Once the seeds have germinated, extract the enzymes from the seeds by grinding them up and mixing them with a buffer solution to create an enzyme extract.
3. Prepare a starch solution by dissolving starch in water to create a starch solution.
4. Divide the starch solution into several test tubes.
5. To each test tube, add a different amount of the enzyme extract obtained from the germinated bean seeds.
6. Incubate the test tubes at a specific temperature for a set amount of time to allow the enzymes to digest the starch.
7. After the incubation period, add iodine solution to each test tube. Iodine reacts with starch to produce a blue-black color. If the enzymes in the germinated bean seed extract have digested the starch, the color of the solution will change from blue-black to a lighter color or become clear.
8. Compare the color changes in the test tubes to determine the extent of starch digestion by the enzymes in the germinated bean seed extract.
By conducting this experiment, one can demonstrate that germinating bean seeds contain enzymes that are capable of digesting starch.