Copy and complete the table by calculating the concentrations and volumes required for each reaction component except H2O and DNA, and calculate the required volumes for the master mix?

| Component | Stock Concentration | Stock Volume (µL) | Final Concentration (µM) | Final Volume (µL) |
|-------------------|---------------------|-------------------|--------------------------|-------------------|
| Forward Primer | 10 µM | 2 | 0.5 | 1 |
| Reverse Primer | 10 µM | 2 | 0.5 | 1 |
| dNTPs | 10 mM | 2 | 200 µM | 1 |
| Taq Polymerase | 5 U/µL | 1 | 0.5 U/µL | 1 |
| 10x Buffer | 10x | 5 | 1x | 1 |
| MgCl2 | 25 mM | 2 | 2.5 mM | 1 |
| Template DNA | N/A | N/A | N/A | N/A |
| H2O | N/A | N/A | N/A | N/A |
| Total Master Mix | N/A | N/A | N/A | 6 |

Table:

| Component | Stock Concentration | Volume Required | |
|---|---|---|
| dNTPs | 10 mM each | |
| Buffer (10X) | 10X | |
| MgCl2 (50 mM) | 50 mM | |
| Primer 1 (100 µM) | 100 µM | |
| Primer 2 (100 µM) | 100 µM | |
| Taq Polymerase | 5 U/µL | |

Calculations:

dNTPs

- Concentration: 0.2 mM each (total 0.8 mM)
- Volume: (0.8 mM / 10 mM) - 100 µL = 8 µL

Buffer (10X)

- Concentration: 1X
- Volume: (1X / 10X) - 100 µL = 10 µL

MgCl2 (50 mM)

- Concentration: 2 mM
- Volume: (2 mM / 50 mM) - 100 µL = 4 µL

Primer 1 (100 µM)

- Volume: 1 µL

Primer 2 (100 µM)

- Volume: 1 µL

Taq Polymerase

- Volume: 0.2 µL (1 U)

Master Mix:

- Total volume: 25 µL
- Volume of master mix required: 25 µL - (8 µL + 10 µL + 4 µL + 1 µL + 1 µL + 0.2 µL) = 0.8 µL